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Derivatization with OPFB


After evaporating the solvent under streaming nitrogen, 200 µl methanol and 200 µl pentafluorobenzylhydroxylamine solution are added. After incubation for 5 min, the mixture is extracted twice each with 1 ml hexane. The combined organic phases are dried under a nitrogen stream. The pentafluorobenzyloximes are re-dissolved in 100 µl acetonitril and stored under argon atmosphere at -20°C until use.

pentafluorobenzylhydroxylamine solution:
50 mM O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (SIGMA) in 1.5 M sodium acetate, pH 4.5

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