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Numer of charts
Methods


GC






Description:

The analysis is carried out using an Agilent 6890 gas chromatograph equipped with a flame ionization detector and a capillary DB-23 column (30 m x 0,25 mm; 0.25 µm coating thickness; J&W Scientific, Agilent). Helium is used as a carrier gas at a flow rate of 1 ml/min. The temperature gradient is as follows: 150 °C for 1 min, 150 – 200 °C at 8 K/min, 200 – 250 °C at 25 K/min and 250 °C for 6 min. For the quantification of esterified fatty acids 200 µg of triheptadecanoate are used as an internal standard.

standards:
standards are available from Sigma (Germany) Larodan (Sweden), Cayman Chemical (USA), kindly provided by Mats Hamberg (Stockholm, Sweden) and Otto Miersch (Halle/Salle, Germany) or enzymatically synthesized, respectively



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Oxylipin Profiling Database All rights reserved 2004 - 2005 A. Schomburg, Plant Biochemistry, University of Goettingen