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Methods


Methylation






Description:

After evaporating the solvent under streaming nitrogen, 400 µl methanol and 6.5 µl trimethylsilyldiazomethane solution are added. The sample is shaken for 30 min and 0.2 µl of acetic acid is added to degrade the remaining trimethylsilyldiazomethane. The sample is dried under streaming nitrogen. The remaining fatty acid methyl esters are re-dissolved in 100 µl acetonitril and stored under argon atmosphere at -20°C until use.

trimethylsilyldiazomethane solution:
2 M trimethylsilyldiazomethane in hexane (SIGMA)



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